PyroCLEAN™

Endotoxin Decontamination

Endotoxin contamination represents a serious problem to both the pharmaceutical and tissue culture industry. Nanogram quantities of this lipopolysaccharides are sufficient to turn multimillion dollar products into non-usable pyrogenic materials driving the cost of manufacture much higher than it should be. Traditional depyrogenation methods include either incineration at 260°C with dry heat or the use of high concentrations of acids or bases for prolonged periods of exposure. Although, these methods are reasonable effective they are extremely expensive, time consuming, cumbersome, and in many cases represent a serious hazard to employees. Additionally, many commonly used materials will not tolerate these harsh conditions, e.g. certain rubbers, plastics, metals, etc.

PyroCLEAN represents a pharmaceutical biodegradable detergent which will solubalize endotoxin so it may be rinsed away with water, alcohol’s or bases, alone or in combination which renders a pharmaceutical surface free of endotoxin. PyroCLEAN will also solubalize many light machine oils and silicone so they may also be rinsed away.

  • PyroCLEAN can also be used to depyrogenate micro and ultrafiltration membranes1 and chromatography columns2.
  • Unlike other detergent based products, PyroCLEAN is traceable down to 4 X10-13g/ml using absorption at 270nm which provides a convenient method of detecting potential residuals.
  • PyroCLEAN is sold as a 4-10X concentrate, depending on application in liter quantities and is available either with or without phenol red indicator for easier visualization on pharmaceutical surfaces.

ALerCHEK, Inc. also offers both endotoxin LAL based testing of materials and endotoxin consulting services. This can take the form of verbal or on site visits if warranted. After years of experience in this specialized area we can save your firm time, money and in many cases we can improve the quality and constancy of your products.


Product Description Cat. # Unit Price
PyroCLEAN 4x Conc. w/indicatorI30103 $185 / L
PyroCLEAN 4x Conc. w/out indicator30103 $185 / L
PyroCLEAN (Bulk) w/indicatorI30104 $875 / 12L
PyroCLEAN (Bulk) w/out indicator30104 $875 / 12L
PyroCLEAN (Bulk) w/ indicatorI30105 $1250 / 5 gal
PyroCLEAN (Bulk) w/out indicator30105 $1250 / 5 gal
Endotoxin Assays (quant. LAL-based)inquire $50 / data point

Free Evaluation Samples and Application Advice Available Upon Request

1. Millipore/Amicon Centrifugal Concentrators (Please ask for product application protocols)
2. Everse, Johannes, Vandegriff, Kim D., and Winslow, Robert M. “Preparation, Purification, and Analysis of Human Hemoglobin”, Methods in Enzymology. Vol. 231, Part B, Biochemical and Analytical Methods